AICS-0088
Protein
proliferating cell nuclear antigen (PCNA)
Gene Symbol
PCNA
Gene Name
proliferating cell nuclear antigen
Structure
DNA replication foci
Certificate of AnalysishPSCreg Certificate

Obtain AICS-0088

Obtain Donor Plasmid

AICS-0088

PCNA in WTC-mEGFP (mono-allelic tag)
Representative media for all clones
Cell line media

Single, mid-level plane of cells in a live hiPS cell colony expressing mEGFP-tagged proliferating cell nuclear antigen (PCNA). Cells were imaged in 3D on a spinning-disk confocal microscope. Scale bar, 5µm.

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NCBI Isoforms:
crRNA Target Site:GTTCGAGGCGCGCCTGGTCC
Linker: GEGQGQGQGPGRGYAYRS
Cas9:Wildtype spCas9
mEGFP Insert
mEGFP Insert
Top: PCNA locus showing 2 PCNA isoforms; Bottom: Zoom in on mEGFP insertion site at PCNA N-terminal exon