About the collection

The Allen Cell Collection contains over one hundred high quality, certified fluorescently tagged hiPSC lines that target dozens of key cellular structures and substructures. These cell lines and their editing plasmids are openly available to academic and commercial researchers through Coriell and Addgene respectively. New lines are released frequently. Subscribe to our newsletter to stay current with out latest releases!

Looking for a line with a disease mutation? Check out our Disease Cell Catalog.

Allen Cell Collection Cell Lines

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Cell Line Catalog

Cell Collection ID
Protein
Gene Symbol & Name
Tagged Alleles
Structure
Fluorescent Tag
Tag Location
Category

AICS-0005

[object Object] thumbnail
paxillin
PXNpaxillin
mono
matrix adhesions
EGFP
C-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0010

[object Object] thumbnail
Sec61 beta
SEC61BSEC61 translocon beta subunit
mono
endoplasmic reticulum
mEGFP
N-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0011

[object Object] thumbnail
Tom20
TOMM20translocase of outer mitochondrial membrane 20
mono
mitochondria
mEGFP
C-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0012

[object Object] thumbnail
alpha-tubulin
TUBA1Btubulin-alpha 1b
mono
microtubules
mEGFP
N-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0013

[object Object] thumbnail
lamin B1
LMNB1lamin B1
mono
nuclear envelope
mEGFP
N-terminus
Key Structure and OrganelleNuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0014

[object Object] thumbnail
fibrillarin
FBLfibrillarin
mono
nucleolus (dense fibrillar component)
mEGFP
C-terminus
Key Structure and OrganelleNuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0016

[object Object] thumbnail
beta-actin
ACTBactin beta
mono
actin filaments
mEGFP
N-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0017

[object Object] thumbnail
desmoplakin
DSPdesmoplakin
mono
desmosomes
mEGFP
C-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0022

[object Object] thumbnail
LAMP-1
LAMP1lysosomal associated membrane protein 1
mono
lysosomes
mEGFP
C-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0023

[object Object] thumbnail
tight junction protein ZO-1
TJP1tight junction protein 1
mono
tight junctions
mEGFP
N-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0024

[object Object] thumbnail
non-muscle myosin heavy chain IIB
MYH10myosin heavy chain 10
mono
actomyosin bundles
mEGFP
N-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0025

[object Object] thumbnail
sialyltransferase 1
ST6GAL1ST6 beta-galactoside alpha-2,6-sialyltransferase 1
mono
Golgi
mEGFP
C-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0025

[object Object] thumbnail
sialyltransferase 1
ST6GAL1ST6 beta-galactoside alpha-2,6-sialyltransferase 1
bi
Golgi
mEGFP
C-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0030

[object Object] thumbnail
autophagy-related protein LC3 B
MAP1LC3Bmicrotubule associated protein 1 light chain 3 beta
mono
autophagosomes
mEGFP
N-terminus
Stress
Obtain Collection
Obtain Plasmid

AICS-0031

[object Object] thumbnail
alpha-tubulin
TUBA1Btubulin-alpha 1b
mono
microtubules
mTagRFP-T
N-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0032

[object Object] thumbnail
centrin-2
CETN2centrin 2
mono
centrioles
mTagRFP-T
N-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0033

[object Object] thumbnail
peroxisomal membrane protein PMP34
SLC25A17solute carrier family 25 member 17
mono
peroxisomes
mEGFP
C-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0034

[object Object] thumbnail
lamin B1
LMNB1lamin B1
mono
nuclear envelope
mTagRFP-T
N-terminus
Key Structure and OrganelleNuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0036

[object Object] thumbnail
CAAX domain of K-Ras
AAVS1safe harbor locus
mono
plasma membrane
mEGFP
NA
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0036

[object Object] thumbnail
CAAX domain of K-Ras
AAVS1safe harbor locus
mono
plasma membrane
mEGFP
NA
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0037

[object Object] thumbnail
troponin I, slow skeletal muscle
TNNI1troponin I1, slow skeletal type
mono
sarcomeric thin filaments
mEGFP
C-terminus
Cardiac Structure
Obtain Collection
Obtain Plasmid

AICS-0040

[object Object] thumbnail
Ras-related protein Rab-5A
RAB5ARAB5A, member RAS oncogene family
bi
endosomes
mEGFP
N-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0040

[object Object] thumbnail
Ras-related protein Rab-5A
RAB5ARAB5A, member RAS oncogene family
mono
endosomes
mEGFP
N-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0046

[object Object] thumbnail
SERCA2
ATP2A2ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
mono
sarcoplasmic reticulum/endoplasmic reticulum
mEGFP
N-terminus
Cardiac Structure
Obtain Collection
Obtain Plasmid

AICS-0048

[object Object] thumbnail
titin
TTNtitin
mono
sarcomeres (M-line tag)
mEGFP
C-terminus
Cardiac Structure
Obtain Collection
Obtain Plasmid

AICS-0052

[object Object] thumbnail
MLC-2a
MYL7myosin light chain 7
mono
sarcomeric thick filaments
mEGFP
C-terminus
Cardiac Structure
Obtain Collection
Obtain Plasmid

AICS-0053

[object Object] thumbnail
connexin-43
GJA1gap junction protein alpha 1
mono
gap junctions
mEGFP
C-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0054

[object Object] thumbnail
CAAX domain of K-Ras
AAVS1safe harbor locus
mono
plasma membrane
mTagRFP-T
NA
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0057

[object Object] thumbnail
nucleophosmin
NPM1nucleophosmin 1
mono
nucleolus (granular component)
mEGFP
C-terminus
Key Structure and OrganelleNuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0058

[object Object] thumbnail
beta-catenin
CTNNB1catenin beta 1
mono
adherens junctions
mEGFP
N-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0059

[object Object] thumbnail
Sec61 betalamin B1
SEC61BSEC61 translocon beta subunit
LMNB1lamin B1
monomono
endoplasmic reticulumnuclear envelope
mEGFPmTagRFP-T
N-terminusN-terminus
Key Structure and OrganelleNuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0060

[object Object] thumbnail
MLC-2v
MYL2myosin light chain 2
mono
sarcomeric thick filaments
mEGFP
C-terminus
Cardiac Structure
Obtain Collection
Obtain Plasmid

AICS-0061

[object Object] thumbnail
histone H2B type 1-J
HIST1H2BJhistone cluster 1 H2B family member j
mono
histones
mEGFP
C-terminus
Nuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0063

[object Object] thumbnail
dystrophin
DMDdystrophin
mono
costameres
mEGFP
C-terminus
Cardiac Structure
Obtain Collection
Obtain Plasmid

AICS-0068

[object Object] thumbnail
SMC protein 1A
SMC1Astructural maintenance of chromosomes 1A
mono
cohesins
mEGFP
C-terminus
Nuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0069

[object Object] thumbnail
nucleoporin Nup153
NUP153nucleoporin 153
mono
nuclear pores
mEGFP
N-terminus
Nuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0074

[object Object] thumbnail
transcription factor SOX-2
SOX2SRY-box transcription factor 2
mono
transcription factor
mEGFP
C-terminus
Nuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0075

[object Object] thumbnail
alpha-actinin-2
ACTN2actinin alpha 2
mono
sarcomeric z-disks
mEGFP
C-terminus
Cardiac Structure
Obtain Collection
Obtain Plasmid

AICS-0078

[object Object] thumbnail
Tom20alpha-tubulin
TOMM20translocase of outer mitochondrial membrane 20
TUBA1Btubulin-alpha 1b
monomono
mitochondriamicrotubules
mEGFPmTagRFP-T
C-terminusN-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0080

[object Object] thumbnail
RNA-binding protein FUS
FUSFUS RNA binding protein
mono
paraspeckles / stress granules
mEGFP
C-terminus
StressNuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0082

[object Object] thumbnail
Ras GTPase-activating protein-binding protein 1 (G3BP-1)
G3BP1G3BP stress granule assembly factor 1
mono
stress granules
mEGFP
N-terminus
Stress
Obtain Collection
Obtain Plasmid

AICS-0083

[object Object] thumbnail
mRNA-decapping enzyme 1A (DCP1A)
DCP1Adecapping mRNA 1A
bi
processing bodies (P-bodies)
mEGFP
N-terminus
Stress
Obtain Collection
Obtain Plasmid

AICS-0083

[object Object] thumbnail
mRNA-decapping enzyme 1A (DCP1A)
DCP1Adecapping mRNA 1A
mono
processing bodies (P-bodies)
mEGFP
N-terminus
Stress
Obtain Collection
Obtain Plasmid

AICS-0084

[object Object] thumbnail
fibrillarinnucleophosmin
FBLfibrillarin
NPM1nucleophosmin 1
monomono
nucleolus (dense fibrillar component)nucleolus (granular component)
mEGFPmTagRFP-T
C-terminusC-terminus
Key Structure and OrganelleNuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0086

[object Object] thumbnail
fibrillarinnucleophosminnucleolar transcription factor UBF
FBLfibrillarin
NPM1nucleophosmin 1
UBTFupstream binding transcription factor
monomonomono
nucleolus (dense fibrillar component)nucleolus (granular component)nucleolus (fibrillar center)
mEGFPmTagRFP-THaloTag
C-terminusC-terminusN-terminus
Key Structure and OrganelleNuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0087

[object Object] thumbnail
mitochondrial transcription factor A (TFAM)
TFAMtranscription factor A, mitochondrial
mono
mitochondrial nucleoids
mEGFP
C-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0088

[object Object] thumbnail
proliferating cell nuclear antigen (PCNA)
PCNAproliferating cell nuclear antigen
mono
DNA replication foci
mEGFP
N-terminus
Nuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0089

[object Object] thumbnail
fibrillarinnucleophosmindCas9-KRAB
FBLfibrillarin
NPM1nucleophosmin 1
CLYBL-dCas9-KRABcitramalyl-CoA lyase safe harbor
monomonomono
nucleolus (dense fibrillar component)nucleolus (granular component)nucleolus (nucleus)
mEGFPmTagRFP-TTagBFP
C-terminusC-terminusNA
Tools
Obtain Collection
Obtain Plasmid

AICS-0090

[object Object] thumbnail
dCas9-KRAB
CLYBL safe harborcitramalyl-CoA lyase safe harbor
mono
nucleus
TagBFP
NA
Tools
Obtain Collection
Obtain Plasmid

AICS-0093

[object Object] thumbnail
telomeric repeat-binding factor 2 (TRF2)
TERF2telomeric repeat binding factor 2
mono
telomeres
mEGFP
N-terminus
Nuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0094

[object Object] thumbnail
SON
SONSON DNA and RNA binding protein
mono
nuclear speckles
mEGFP
N-terminus
Nuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0095

[object Object] thumbnail
EZH2
EZH2enhancer of zeste 2 polycomb repressive complex 2 subunit
mono
polycomb repressive complex 2
mEGFP
N-terminus
Nuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0096

[object Object] thumbnail
RPB1
POLR2ARNA polymerase II subunit A
mono
RNA polymerase II
mEGFP
N-terminus
Nuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0099

[object Object] thumbnail
CCCTC-binding factor (CTCF)
CTCFCCCTC-binding factor
mono
CTCF-binding sites
mEGFP
N-terminus
Nuclear Structure
Obtain Collection
Obtain Plasmid

AICS-0102

[object Object] thumbnail
CAAX domain of K-Ras
AAVS1safe harbor locus
mono
plasma membrane
mTagRFP-T
NA
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0114

[object Object] thumbnail
E-cadherin
CDH1cadherin 1
bi
adherens junctions
mEGFP
C-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0114

[object Object] thumbnail
E-cadherin
CDH1cadherin 1
mono
adherens junctions
mEGFP
C-terminus
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0120

[object Object] thumbnail
Tom20CAAX domain of K-Ras
TOMM20translocase of outer mitochondrial membrane 20
AAVS1safe harbor locus
monomono
mitochondriaplasma membrane
mEGFPmTagRFP-T
C-terminusNA
Key Structure and Organelle
Obtain Collection
Obtain Plasmid

AICS-0126

[object Object] thumbnail
Vascular endothelial (VE)-cadherin
CDH5cadherin 5
bi
endothelial adherens junctions
mEGFP
C-terminus
Endothelial
Obtain Collection
Obtain Plasmid

AICS-0126

[object Object] thumbnail
Vascular endothelial (VE)-cadherin
CDH5cadherin 5
mono
endothelial adherens junctions
mEGFP
C-terminus
Endothelial
Obtain Collection
Obtain Plasmid

Cell Line Catalog

Coming soon
Cell Collection IDProteinGene Symbol & NameTagged AllelesStructureFluorescent TagTag LocationCategory

AICS-0122

T-box protein
EOMESeomesodermin
transcription factor
mEGFP
C-terminus

Acknowledgements

The Allen Institute for Cell Science also acknowledges the following people for their expertise and support:

Bruce R. Conklin, Gladstone Institute and University of California, San Francisco
Charles E. Murray, University of Washington
William C. Skarnes, Wellcome Trust Sanger Institute
David Drubin, University of California, Berkley

Funding

The generation of some of these cell lines was supported by the National Human Genome Research Institute of the National Institutes under Award Number UM1HG011593.

We wish to thank Allen Institute founders, Jody Allen & Paul G. Allen, for their vision, encouragement, and support.